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Proceedings Paper

Investigating the structural changes of β-amyloid peptide aggregation using attenuated-total-reflection surface-enhanced Raman spectroscopy
Author(s): K.-C. Chiu; L.-Y. Yu; J.-N. Yih; S.-J. Chen
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Paper Abstract

This study utilizes a surface-enhanced Raman spectroscopy (SERS) based on the attenuated-total-reflection (ATR) method to investigate that the structural information of the biomolecular monolayer on sensing surface can be dynamically observed with a higher signal-to-noise ratio signal. The secondary structures of long oligonucleotides and their influence on the DNA hybridization on the sensing surface are investigated. The SERS spectrum provides the structural information of the oligonucleotides with the help of a silver colloidal nanoparticle monolayer by control of the size and distribution of the nanoparticles adapted as a Raman active substrate. It is found that the ring-breathing modes of adenine, thymine, guanine, and cytosine in Raman fingerprint associated with three 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35°C to 45°C reduces secondary structure effects. The ATR-SERS biosensing technique will be used to provide valuable structural information regarding the short-term reversible interactions and long-term polymerization events in the A&bgr; aggregates on the sensing surface.

Paper Details

Date Published: 14 February 2007
PDF: 8 pages
Proc. SPIE 6450, Plasmonics in Biology and Medicine IV, 64500R (14 February 2007); doi: 10.1117/12.701757
Show Author Affiliations
K.-C. Chiu, National Cheng Kung Univ. (Taiwan)
L.-Y. Yu, National Cheng Kung Univ. (Taiwan)
J.-N. Yih, National Kaohsiung Univ. of Applied Science (Taiwan)
S.-J. Chen, National Cheng Kung Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6450:
Plasmonics in Biology and Medicine IV
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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