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Proceedings Paper

Monitoring Pc 4-mediated photodynamic therapy of U87 tumors with 18F- fluorodeoxy-glucose PET imaging in the Athymic Nude Rat
Author(s): Davood Varghai; Nathan Cross; Chandra Spring-Robinson; Rahul Sharma; Denise K. Feyes; Yusra Ahmad; Nancy L. Oleinick; Raymond F. Muzic; David Dean
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Paper Abstract

Introduction: We have previously demonstrated the use of phthalocyanine Pc 4 for the photodynamic therapy (PDT) of ectopic human glial tumors in the athymic nude rat brain. We wish to determine whether 18F-fluorodeoxy-glucose (18F-FDG) Positron Emission Tomography (PET) imaging can detect the reduction in tumor metabolism that must occur after Pc 4-PDT-induced necrosis. Methods: 2.5 x 105 U87 cells were injected into the brains of 12 athymic nude rats. After 7 days of tumor growth, all 12 animals were imaged functionally by 18F-FDG micro-PET (&mgr;PET) and structurally by micro-CT and/or micro-MR. These animals received 0.5 mg/kg b.w. Pc 4 via tail-vein injection. One day later the scalp was re-incised and the tumor illuminated with 30 J/cm2 of 672-nm light from a diode laser. The next day these animals were again 18F-FDG &mgr;PET imaged. Next, the animals were euthanized and their brains were explanted for H&E histology. Results: Histology showed that tumors in the 6 Pc 4-PDT-treated animals demonstrated necrosis ranging from full to frank (severe). Preliminary analysis showed that 18F-FDG &mgr;PET activity in 3 of the 6 non-PDT group (i.e., no tumor necrosis observed) animals was seen to increase 2.28 times following tumor photoirradiation, whereas 18F-FDG &mgr;PET activity in 5 of the 6 PDT group (i.e., tumor necrosis observed) animals was seen to increase 1.15 times following tumor photoirradiation. Discussion: The increased 18F-FDG &mgr;PET activity in the PDT group was unexpected. We had expected this activity to decrease and are presently investigating the cause of this observation.

Paper Details

Date Published: 23 March 2007
PDF: 7 pages
Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64242G (23 March 2007); doi: 10.1117/12.701517
Show Author Affiliations
Davood Varghai, Case Western Reserve Univ. (United States)
Nathan Cross, Case Western Reserve Univ. (United States)
Chandra Spring-Robinson, Case Western Reserve Univ. (United States)
Rahul Sharma, Case Western Reserve Univ. (United States)
Denise K. Feyes, Case Western Reserve Univ. (United States)
Yusra Ahmad, Case Western Reserve Univ. (United States)
Nancy L. Oleinick, Case Western Reserve Univ. (United States)
Raymond F. Muzic, Case Western Reserve Univ. (United States)
David Dean, Case Western Reserve Univ. (United States)

Published in SPIE Proceedings Vol. 6424:
Photonic Therapeutics and Diagnostics III
Henry Hirschberg; Steen J. Madsen; Brian Jet-Fei Wong; Justus F. R. Ilgner; Reza S. Malek; Kenton W. Gregory; Guillermo J. Tearney; Nikiforos Kollias; Bernard Choi; Haishan Zeng, Editor(s)

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