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Proceedings Paper

Usefulness and limitation of measurement methods for evaluation of tissue-engineered cartilage function and characterization using nanosecond pulsed laser
Author(s): Miya Ishihara; Masato Sato; Nagatoshi Kaneshiro; Genya Mitani; Toshihiro Nagai; Toshiharu Kutsuna; Masayuki Ishihara; Joji Mochida; Makoto Kikuchi
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Paper Abstract

There is a demand in the field of regenerative medicine for measurement technology that enables determination of functions and characterizations of engineered tissue. Regenerative medicine involving the articular cartilage in particular requires measurement of viscoelastic properties and characterization of the extracellular matrix, which plays a major role in articular cartilage. To meet this demand, we previously proposed a noninvasive method for determination of the viscoelasticity using laser-induced thermoelastic wave (1,2). We also proposed a method for characterization of the extracellular matrix using time-resolved autofluorescence spectroscopy, which could be performed simultaneously with laser-induced thermoelastic wave measurement(3). The purpose of this study was to verify the usefulness and limitation of these methods for evaluation of actual engineered cartilage. 3rd Q-SW Nd:YAG laser pulses, which are delivered through optical fiber, were used for the light source. Laser-induced thermoelastic waves were detected by a sensor consisting of a piezoelectric transducer, which was designed for use in arthroscopy(4). The time-resolved fluorescence spectroscopy was measured by a photonic multichannel analyzer with 4ch digital signal generator. Various tissue-engineered cartilages were developed as samples. Only a limited range of sample thickness could be measured, however, the measured viscoelastic parameters had a positive correlation with culture time, that is, the degree of formation of extracellular matrix(5,6). There were significant differences in the fluorescent parameters among the phenotypic expressions of cartilage because chondrocyte produces specific extracellular matrix as in collagen types depending on its phenotype.

Paper Details

Date Published: 12 February 2007
PDF: 4 pages
Proc. SPIE 6439, Optics in Tissue Engineering and Regenerative Medicine, 643909 (12 February 2007); doi: 10.1117/12.701489
Show Author Affiliations
Miya Ishihara, National Defense Medical College (Japan)
Tokai Univ. School of Medicine (Japan)
Masato Sato, Tokai Univ. School of Medicine (Japan)
Nagatoshi Kaneshiro, Tokai Univ. School of Medicine (Japan)
Genya Mitani, Tokai Univ. School of Medicine (Japan)
Toshihiro Nagai, Tokai Univ. School of Medicine (Japan)
Toshiharu Kutsuna, Tokai Univ. School of Medicine (Japan)
Masayuki Ishihara, National Defense Medical College Research Institute (Japan)
Joji Mochida, Tokai Univ. School of Medicine (Japan)
Makoto Kikuchi, National Defense Medical College Research Institute (Japan)
National Defense Medical College (Japan)


Published in SPIE Proceedings Vol. 6439:
Optics in Tissue Engineering and Regenerative Medicine
Sean J. Kirkpatrick; Ruikang K. Wang, Editor(s)

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