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Proceedings Paper

Laser induced fluorescence as a diagnostic tool integrated into a scanning fiber endoscope for mouse imaging
Author(s): Christopher M. Brown; Lillian Maggio-Price; Eric J. Seibel
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Paper Abstract

Scanning fiber endoscope (SFE) technology has shown promise as a minimally invasive optical imaging tool. To date, it is capable of capturing full-color 500-line images, at 15 Hz frame rate in vivo, as a 1.6 mm diameter endoscope. The SFE uses a singlemode optical fiber actuated at mechanical resonance to scan a light spot over tissue while backscattered or fluorescent light at each pixel is detected in time series using several multimode optical fibers. We are extending the capability of the SFE from a RGB reflectance imaging device to a diagnostic tool by imaging laser induced fluorescence (LIF) in tissue, allowing for correlation of endogenous fluorescence to tissue state. Design of the SFE for diagnostic imaging is guided by a comparison of single point spectra acquired from an inflammatory bowel disease (IBD) model to tissue histology evaluated by a pathologist. LIF spectra were acquired by illuminating tissue with a 405 nm light source and detecting intrinsic fluorescence with a multimode optical fiber. The IBD model used in this study was mdr1a-/- mice, where IBD was modulated by infection with Helicobacter bilis. IBD lesions in the mouse model ranged from mild to marked hyperplasia and dysplasia, from the distal colon to the cecum. A principle components analysis (PCA) was conducted on single point spectra of control and IBD tissue. PCA allowed for differentiation between healthy and dysplastic tissue, indicating that emission wavelengths from 620 - 650 nm were best able to differentiate diseased tissue and inflammation from normal healthy tissue.

Paper Details

Date Published: 15 February 2007
PDF: 12 pages
Proc. SPIE 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, 64330M (15 February 2007); doi: 10.1117/12.701386
Show Author Affiliations
Christopher M. Brown, Univ. of Washington (United States)
Lillian Maggio-Price, Univ. of Washington (United States)
Eric J. Seibel, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 6433:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII
Israel Gannot, Editor(s)

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