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Proceedings Paper

Phantom and in-vivo measurements of hemoglobin concentration and oxygen saturation using PCT-S small animal scanner
Author(s): Bo Liu; Daniel Reinecke; Robert A. Kruger; Keith M. Stantz
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Paper Abstract

Purpose: The purpose of this study is to calibrate the PCT small animal scanner system with a blood phantom and to measure the blood hemoglobin concentration and oxygen saturation level in mouse tail vein and tumors. Methods and Materials: A blood phantom with variable blood flow and oxygen content was integrated into the PCT scanner with a circulation system. The circulation system consisted of a pump, an oxygen electrode detector and a tonometer. The SaO2 values were determined based on a linear combination model of oxy-hemoglobin and deoxy-hemoglobin absorption spectrum curves. Hemoglobin concentration (CHb) was determined by measuring the linear relationship for different blood dilutions. SaO2 and CHb as measured in vivo for the artery and vein in a mouse tail were also measured. Results: The PCT spectra of blood phantom samples were measured and compared with hemoglobin optical absorption spectra. The linear relationship between hemoglobin concentration and PCT intensities were observed by phantom study. The hemoglobin concentration of mouse is ~9.7g/dL. The saturation difference between arteries and veins in mouse tail is also measured by PCT scan. Conclusions: Both the phantom and living mouse tail vessel scans have shown that NIR PCT-spectroscopy can be used to measure the hemoglobin saturation level and hemoglobin concentration in small animal for future tumor hypoxia study.

Paper Details

Date Published: 13 February 2007
PDF: 9 pages
Proc. SPIE 6437, Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 64371X (13 February 2007); doi: 10.1117/12.701376
Show Author Affiliations
Bo Liu, Purdue Univ. (United States)
Daniel Reinecke, Optosonics, Inc. (United States)
Robert A. Kruger, Optosonics, Inc. (United States)
Keith M. Stantz, Purdue Univ. (United States)
Indiana Univ. (United States)


Published in SPIE Proceedings Vol. 6437:
Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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