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Proceedings Paper

Characterization of cell samples from measurements of spectroscopic scattering phase-dispersion
Author(s): Shellee D. Dyer; Lara K. Street; Shelley M. Etzel; Tasshi Dennis; Andrew Dienstfrey; Vadim Tsvankin; Wei Tan
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Paper Abstract

We present spectroscopic swept-source optical coherence tomography (OCT) measurements of the phase-dispersion of cell samples. We have previously demonstrated that the phase of the scattered field is, in general, independent of the intensity, and both must be measured for a complete characterization of the sample. In this paper, we show that, in addition to providing a measurement of the size of the cell nuclei, the phase spectrum provides a very sensitive indication of the separations between the cells. Epithelial cancers are characterized by many factors, including enlarged nuclei and a significant loss in the architectural orientation of the cells. Therefore, an in vivo diagnostic tool that analyzes multiple properties of the sample instead of focusing on cellular nuclei sizes alone could provide a better assessment of tissue health. We show that the phase spectrum of the scattered light appears to be more sensitive to cell spacing than the intensity spectrum. It is possible to determine simultaneously the cell nuclei sizes from the intensity spectrum and the nuclei spacing from the phase spectrum. We measure cell monolayer samples with high and low cell density and compare measured results with histograms of the cell separations calculated from microscope images of the samples. We show qualitative agreement between the predicted histograms and the interferometric results.

Paper Details

Date Published: 16 February 2007
PDF: 8 pages
Proc. SPIE 6446, Biomedical Applications of Light Scattering, 64460V (16 February 2007); doi: 10.1117/12.701239
Show Author Affiliations
Shellee D. Dyer, National Institute of Standards and Technology (United States)
Lara K. Street, National Institute of Standards and Technology (United States)
Shelley M. Etzel, National Institute of Standards and Technology (United States)
Tasshi Dennis, National Institute of Standards and Technology (United States)
Andrew Dienstfrey, National Institute of Standards and Technology (United States)
Vadim Tsvankin, Univ. of Colorado/Boulder (United States)
Wei Tan, Univ. of Colorado/Boulder (United States)

Published in SPIE Proceedings Vol. 6446:
Biomedical Applications of Light Scattering
Adam Wax; Vadim Backman, Editor(s)

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