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Proceedings Paper

Fluorescence lifetime contrast in small animal imaging
Author(s): V. Krishnan Ramanujan; Abhik Bandyopadhyay; Luzhe Sun; Brian A. Herman
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Paper Abstract

Early detection of primary tumors is the key for effective therapeutic intervention and successful patient survival. Small animal models emulating human diseases are powerful tools for our comprehensive understanding of the pathophysiology of tumor formation and metastasis to distant sites. Our long-term goal is to develop a non-invasive, multiphoton-fluorescence lifetime imaging (MP-FLIM) modality that can precisely quantify these steps in animal tumor models at a very early stage. The specific hypothesis is that fluorescence lifetime can be employed as reliable contrast parameter for providing higher detection sensitivity as compared with conventional intensity-based tumor imaging approaches and therefore it is possible to detect smaller tumor volumes (early detection) than those achieved by other prevailing methods. We base this hypothesis on our recent observations that (1) fluorescence lifetime is "intrinsic" to the fluorophore and its measurement is not affected by concentration and/or spectral artifacts as in intensity-based methods, (2) multiphoton excitation can enable increased tissue penetrability and reduced phototoxicity and (3) MP-FLIM approach can discriminate background autofluorescence from the fluorescent proteins in thick tissues thereby achieving a ten-fold increase in signal-to-background ratio over the intensity-based approaches. We present our preliminary data to support this hypothesis in primary tumor detection in nu/nu athymic mouse models.

Paper Details

Date Published: 14 February 2007
PDF: 4 pages
Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 644911 (14 February 2007); doi: 10.1117/12.701133
Show Author Affiliations
V. Krishnan Ramanujan, Univ. of Texas Health Science Ctr. at San Antonio (United States)
Abhik Bandyopadhyay, Univ. of Texas Health Science Ctr. at San Antonio (United States)
Luzhe Sun, Univ. of Texas Health Science Ctr. at San Antonio (United States)
Brian A. Herman, Univ. of Texas Health Science Ctr. at San Antonio (United States)


Published in SPIE Proceedings Vol. 6449:
Genetically Engineered and Optical Probes for Biomedical Applications IV
Samuel Achilefu; Alexander Pavlovich Savitsky; Rebekka M. Wachter; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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