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Proceedings Paper

Correlation of contrast-enhanced MR images with the histopathology of minimally invasive thermal and cryoablation cancer treatments in normal dog prostates
Author(s): D. M. Bouley; B. Daniel; K. Butts Pauly; E. Liu; A. Kinsey; W. Nau; C. J. Diederich; G. Sommer
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Paper Abstract

Magnetic Resonance Imaging (MRI) is a promising tool for visualizing the delivery of minimally invasive cancer treatments such as high intensity ultrasound (HUS) and cryoablation. We use an acute dog prostate model to correlate lesion histopathology with contrast-enhanced (CE) T1 weighted MR images, to aid the radiologists in real time interpretation of in vivo lesion boundaries and pre-existing lesions. Following thermal or cryo treatments, prostate glands are removed, sliced, stained with the vital dye triphenyl tetrazolium chloride, photographed, fixed and processed in oversized blocks for routine microscopy. Slides are scanned by Trestle Corporation at .32 microns/pixel resolution, the various lesions traced using annotation software, and digital images compared to CE MR images. Histologically, HUS results in discrete lesions characterized by a "heat-fixed" zone, in which glands subjected to the highest temperatures are minimally altered, surrounded by a rim or "transition zone" composed of severely fragmented, necrotic glands, interstitial edema and vascular congestion. The "heat-fixed" zone is non-enhancing on CE MRI while the "transition zone" appears as a bright, enhancing rim. Likewise, the CE MR images for cryo lesions appear similar to thermally induced lesions, yet the histopathology is significantly different. Glands subjected to prolonged freezing appear totally disrupted, coagulated and hemorrhagic, while less intensely frozen glands along the lesion edge are partially fragmented and contain apoptotic cells. In conclusion, thermal and cryo-induced lesions, as well as certain pre-existing lesions (cystic hyperplasia - non-enhancing, chronic prostatitis - enhancing) have particular MRI profiles, useful for treatment and diagnostic purposes.

Paper Details

Date Published: 8 February 2007
PDF: 12 pages
Proc. SPIE 6440, Thermal Treatment of Tissue: Energy Delivery and Assessment IV, 644006 (8 February 2007); doi: 10.1117/12.701049
Show Author Affiliations
D. M. Bouley, Stanford Univ. (United States)
B. Daniel, Stanford Univ. (United States)
K. Butts Pauly, Stanford Univ. (United States)
E. Liu, Stanford Univ. (United States)
A. Kinsey, Univ. of California, San Francisco (United States)
W. Nau, Univ. of California, San Francisco (United States)
C. J. Diederich, Univ. of California, San Francisco (United States)
G. Sommer, Stanford Univ. (United States)


Published in SPIE Proceedings Vol. 6440:
Thermal Treatment of Tissue: Energy Delivery and Assessment IV
Thomas P. Ryan, Editor(s)

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