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Proceedings Paper

To see the unseeable: confocal miniprobes for routine microscopic imaging during endoscopy
Author(s): A. Osdoit; F. Lacombe; Charlotte Cavé; S. Loiseau; E. Peltier
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Paper Abstract

Confocal fluorescence high resolution imaging during standard endoscopic procedures has been presented as a very promising tool to enhance patient care and physician practice by providing supplementary diagnostic information in real-time. The purpose of this paper is to show not only potential, but convincing results of endoscopic microscopy using a catheter-based approach. Mauna Kea Technologies' core technology, Cellvizio, delivers dynamic imaging at 12 frames/second using confocal miniprobes inserted through the operating channel of regular endoscopes. Cellvizio is composed of 3 parts including (a) a Laser Scanning Unit, (b) Confocal MiniprobeTM with the following characteristics: 5-15 &mgr;m axial resolution, 2-5 &mgr;m lateral resolution, 15-100 &mgr;m depth of penetration, field of view of 600x500 &mgr;m and (c) a software package with onthe- fly processing capabilities. With several tens of patients examined during routine GI endoscopy procedures, the most relevant clinical parameters could be assessed in a doubled-blinded fashion between the endoscopist and a pathologist and results showing very high accuracy in the differentiation of neoplasia from normal and hyperplastic tissue were obtained. In the field of pulmonology, the micro-autofluorescence properties of tissues could be assessed and structures never before accessed in vivo were observed. Cellvizio® may be useful to study bronchial remodeling in asthma and chronic obstructive pulmonary diseases. Using appropriate topical fluorescent dye, the Confocal Miniprobes may also make it possible to perform optical biopsy of precancerous and superficial bronchial cancers. Cellvizio® is as a new tool towards "targeted biopsies", leading to earlier, more reliable and cost effective diagnostic procedures. Other applications, specifically in molecular imaging are also made possible by the miniaturization of the probe (combination with biopsy needle for solid organs use or lymph node detection) and by the compatibility of the system with other imaging modalities (auto-fluorescence and narrow-band imaging endoscopy, MRI, PET, etc).

Paper Details

Date Published: 14 February 2007
PDF: 9 pages
Proc. SPIE 6432, Endoscopic Microscopy II, 64320F (14 February 2007); doi: 10.1117/12.701004
Show Author Affiliations
A. Osdoit, Mauna Kea Technologies (France)
F. Lacombe, Mauna Kea Technologies (France)
Charlotte Cavé, Mauna Kea Technologies (France)
S. Loiseau, Mauna Kea Technologies (France)
E. Peltier, Mauna Kea Technologies (France)


Published in SPIE Proceedings Vol. 6432:
Endoscopic Microscopy II
Guillermo J. Tearney; Thomas D. Wang, Editor(s)

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