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Proceedings Paper

Microscopy using micropixellated light emitting diodes
Author(s): V. Poher; G. T. Kennedy; D. S. Elson; P. M. W. French; M. A. A. Neil; H. Z. Zhang; E. Gu; Z. Gong; C. Griffin; J. M. Girkin; M. D. Dawson
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Paper Abstract

In this paper we demonstrate structured illumination microscopy, a cheap and flexible method of obtaining optical sectioning in wide-field microscopy, and we investigate line-scanning microscopy, both using the same microelement LED. In our first experiment we demonstrate structured illumination using a custom-designed microelement LED consisting of 120 individually addressable stripes emitting at 470 nm. An electrical driver was designed to produce a programmable grid pattern and the device was located in an Olympus BX41 fluorescence microscope in critical illumination configuration. By using an integrated solid-state alternative to a conventional piezo-actuated grid and separate illumination source, we improved the speed and accuracy of the system, reducing the artefacts due to the errors in the grid positions. Additionally, we investigated the use of the same LED device as a scanning source for confocal line-scanning microscopy. After each line scanning, an image was acquired using a CCD camera and the out-of-focus light was rejected by a post-processing method.

Paper Details

Date Published: 14 February 2007
PDF: 5 pages
Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 644313 (14 February 2007); doi: 10.1117/12.700811
Show Author Affiliations
V. Poher, Imperial College London (United Kingdom)
G. T. Kennedy, Imperial College London (United Kingdom)
D. S. Elson, Imperial College London (United Kingdom)
P. M. W. French, Imperial College London (United Kingdom)
M. A. A. Neil, Imperial College London (United Kingdom)
H. Z. Zhang, Univ. of Strathclyde (United Kingdom)
E. Gu, Univ. of Strathclyde (United Kingdom)
Z. Gong, Univ. of Strathclyde (United Kingdom)
C. Griffin, Univ. of Strathclyde (United Kingdom)
J. M. Girkin, Univ. of Strathclyde (United Kingdom)
M. D. Dawson, Univ. of Strathclyde (United Kingdom)

Published in SPIE Proceedings Vol. 6443:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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