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Proceedings Paper

Selective cellular delivery of self-assembled quantum dot-peptide bioconjugates
Author(s): James B. Delehanty; Igor L. Medintz; Thomas Pons; Philip E. Dawson; Florence M. Brunel; Hedi Mattoussi
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Paper Abstract

We demonstrate the selective delivery of self-assembled luminescent semiconductor quantum dot (QD)-peptide bioconjugates into several eukaryotic cell lines. A 23-mer hetero-bifunctional peptide bearing a positively-charged oligoarginine domain and a terminal polyhistidine tract was synthesized and used to mediate the cellular internalization of the QD-bioconjugates. The polyhistidine tract allows the peptide to self-assemble onto the QD surface via metal-ion coordination while the oligoarginine domain mediates the specific uptake of the QD-bioconjugates via electrostatic interactions with cell surface receptors. In both HEK 293T/17 and COS-1 cells, this peptide-mediated delivery is concentration-dependent in terms of both the QD concentration and the peptide:QD ratio. Intracellularly, the QD signal is punctate in appearance and some, but not all, of the QDs are located within recycling endosomes as evidenced by their colocalization with transferrin. In both cell lines, the QD-bioconjugates elicit minimal cytotoxicity within the timeframe required for adequate cellular uptake. The specificity of this delivery strategy is demonstrated by performing a multicolor QD labeling, wherein the presence or absence of the peptide on the QD surface controls cellular uptake.

Paper Details

Date Published: 27 February 2007
PDF: 10 pages
Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480U (27 February 2007); doi: 10.1117/12.700768
Show Author Affiliations
James B. Delehanty, U.S. Naval Research Lab. (United States)
Igor L. Medintz, U.S. Naval Research Lab. (United States)
Thomas Pons, U.S. Naval Research Lab. (United States)
Johns Hopkins Univ. (United States)
Philip E. Dawson, The Scripps Research Institute (United States)
Florence M. Brunel, The Scripps Research Institute (United States)
Hedi Mattoussi, U.S. Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 6448:
Colloidal Quantum Dots for Biomedical Applications II
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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