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Proceedings Paper

Observation of cell dynamics by laser scanning Raman microscope
Author(s): Keisaku Hamada; Katsumasa Fujita; Minoru Kobayashi; Satoshi Kawata
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Paper Abstract

We developed a Raman microscope using a slit-scanning technique for observation of biological samples. A sample was illuminated by a line-shaped laser light, and Raman spectra were measured at different points in the line simultaneously by a spectrometer equipped with a 2D detector. The parallel detection of the Raman spectra boosts the image acquisition rate, which enable us to observe a living biological sample with high temporal and spatial resolution. We also applied a noise reduction technique using singular value decomposition. We recorded motion of intracellular components of living HeLa cells as sequential Raman images in a spectral region between 600 - 3000 cm-1 with the temporal resolution of 3 minutes.

Paper Details

Date Published: 14 February 2007
PDF: 6 pages
Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430Z (14 February 2007); doi: 10.1117/12.700729
Show Author Affiliations
Keisaku Hamada, Osaka Univ. (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)
Minoru Kobayashi, Nanophoton Corp. (Japan)
Satoshi Kawata, Osaka Univ. (Japan)
RIKEN (Japan)

Published in SPIE Proceedings Vol. 6443:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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