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Proceedings Paper

Correlation between cell viability and cumulative singlet oxygen luminescence from protoporphyrin IX in varying subcellular localizations
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Paper Abstract

Photodynamic therapy (PDT) can be targeted toward different subcellular localizations and it is widely believed different subcellular targets vary in their sensitivity to photobiological damage. In this study, PDT-generated near-infrared singlet oxygen (1O2) luminescence was measured along with cell viability under two different incubation protocols: 5- aminolevulinic acid (ALA) endogenously-induced protoporphyrin IX (PpIX) and exogenous PpIX, at different incubation times. Confocal fluorescence microscopy indicated that ALA-induced PpIX (2 h) localized in the mitochondria, whereas exogenous PpIX (1 h) mainly localized to the plasma membrane. Cell viability was determined at several time points during PDT treatments using colony-forming assays, and the surviving fraction correlated well with cumulative 1O2 luminescence counts under both incubation protocols. Preliminary results indicate the plasma membrane is less sensitive to PDT-generated 1O2 than the mitochondria.

Paper Details

Date Published: 27 February 2007
PDF: 9 pages
Proc. SPIE 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI, 642707 (27 February 2007); doi: 10.1117/12.700674
Show Author Affiliations
Buhong Li, Ontario Cancer Institute (Canada)
Univ. of Toronto (Canada)
Fujian Normal Univ. (China)
Mark T. Jarvi, Ontario Cancer Institute (Canada)
Univ. of Toronto (Canada)
Eduardo H. Moriyama, Ontario Cancer Institute (Canada)
Univ. of Toronto (Canada)
Brian C. Wilson, Ontario Cancer Institute (Canada)
Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 6427:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI
David Kessel, Editor(s)

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