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Proceedings Paper

Study of normal, fibrous, and calcified aortic valve tissue by Raman and reflectance spectroscopy
Author(s): Kátia Calligaris Rodrigues; Egberto Munin; Leandro P. Alves; Fabrício L. Silveira; Landulfo S. Junior; Carlos J. De Lima; João C. Lázzaro; Genivaldo C. De Souza; José A. B. Piotto; Marcos T. T. Pacheco; Renato A. Zângaro
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Paper Abstract

Several studies have identified the degree of aortic valve calcification as a strong predictor both for the progression and outcome of aortic stenosis. In industrialized countries, aortic valve stenosis is most frequently caused by progressive calcification and degeneration of aortic cusps. However, there are no accurate methods to quantify the extent of aortic valve calcification. To provide a non-invasive alternative to biopsy, a range of optical methods have been investigated, including Raman and reflectance spectroscopy. A Raman spectrum can be used to access the molecular constitution of a particular tissue and classify it. Raman spectroscopy is largely used in the quantification and evaluation of human atherosclerosis, being a powerful technique for performing biochemical analysis without tissue removal. Nevertheless, increased thickness and disorganization of the collagen fibre network and extracellular matrix are known to affect the diffuse spectral reflectance of the tissue. A catheter with the "6 around 1" configuration, the central fiber transmit laser radiation to the sample and the scattered light is collected by the other six surrounding fibers, was used both for Raman and reflectance spectroscopy. A white light (krypton lamp, flashtube Model FX 1160 Perkin Elmer, USA) excitation was used for reflectance measurements. A Ti-sapphire (785nm, Spectra Physics, model 3900S, USA) laser, pumped by an argon laser (Spectra Physics, model Stabilite 2017, USA) was used as the near infrared Raman set up. Several ex-vivo spectra of aortic valve samples were analyzed. The results show a promising way to differentiate normal, fibrous and calcified tissue in aortic valve.

Paper Details

Date Published: 23 March 2007
PDF: 8 pages
Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64241U (23 March 2007); doi: 10.1117/12.700645
Show Author Affiliations
Kátia Calligaris Rodrigues, Vale do Paraíba Univ. (Brazil)
Egberto Munin, Vale do Paraíba Univ. (Brazil)
Leandro P. Alves, Vale do Paraíba Univ. (Brazil)
Fabrício L. Silveira, Vale do Paraíba Univ. (Brazil)
Landulfo S. Junior, Vale do Paraíba Univ. (Brazil)
Carlos J. De Lima, Vale do Paraíba Univ. (Brazil)
João C. Lázzaro, Vale do Paraíba Univ. (Brazil)
Genivaldo C. De Souza, Cirucor Clinic (Brazil)
José A. B. Piotto, Cirucor Clinic (Brazil)
Marcos T. T. Pacheco, Vale do Paraíba Univ. (Brazil)
Renato A. Zângaro, Vale do Paraíba Univ. (Brazil)


Published in SPIE Proceedings Vol. 6424:
Photonic Therapeutics and Diagnostics III
Henry Hirschberg; Brian Jet-Fei Wong; Reza S. Malek; Kenton W. Gregory; Nikiforos Kollias; Bernard Choi; Steen J. Madsen; Guillermo J. Tearney; Justus F. R. Ilgner; Haishan Zeng, Editor(s)

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