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Proceedings Paper

Programmable diffractive optics for laser scanning confocal microscopy
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Paper Abstract

In this work, we describe the design and implementation of a laser scanning confocal microscope with active beam forming optics. We demonstrate dynamic control over intensity and polarization properties of the beam using the technique of programmable diffractive optics. This technique is used facilitate active aberration correction in the beam and to generate radially polarized pupil function in order to get an on-axis axially polarized point spread function. We also develop the high numerical aperture theory to calculate the focal point spread function for a radially polarized pupil function. We describe the design and implementation of a simple vector beam formation unit consisting of a polarizing beam splitter and two right angled prisms in conjunction with a ferro-electric spatial light modulator. We also describe the design and implementation of a beam scanning system comprising of a novel off-axis scanner mirror that maintains registration of the conjugate pupil planes in the system.

Paper Details

Date Published: 14 February 2007
PDF: 10 pages
Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 644310 (14 February 2007); doi: 10.1117/12.700611
Show Author Affiliations
B. R. Boruah, Imperial College London (United Kingdom)
M. A. A. Neil, Imperial College London (United Kingdom)


Published in SPIE Proceedings Vol. 6443:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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