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Proceedings Paper

A novel hyperspectral lifetime probe for autofluorescence
Author(s): Pieter De Beule; C. Dunsby; D. M. Owen; N. P. Galletly; U. Anand; C. D. Benham; A. Naylor; G. W. Stamp; P. Anand; P. M. W. French
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Paper Abstract

The application of autofluorescence in non-invasive medical diagnostics could have great potential. Two major drawbacks inherent to this approach are low signal levels compared to those from exogenous fluorescent probes and complexity caused by the multiplicity of fluorescent biomolecules in tissue. Here we present a new optical system that is based on single channel detection via an optical fiber and can measure the fluorescence emission spectrum and fluorescence lifetime simultaneously for excitation wavelengths of 355 and 435nm. Single channel measurements integrate the signal normally available in an imaging setup and therefore have a better signal-tonoise ratio. Resolving both the fluorescence emission spectrum and fluorescence lifetime provides the opportunity to discriminate multiple fluorophores. This instrument is intended for NAD(P)H and flavin measurements for the dynamic monitoring of cellular metabolism and optical measurements of cancerous tissue. Initial results from a study of live cells and a clinical study of human skin lesions are presented.

Paper Details

Date Published: 14 February 2007
PDF: 9 pages
Proc. SPIE 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, 643303 (14 February 2007); doi: 10.1117/12.700590
Show Author Affiliations
Pieter De Beule, Imperial College London (United Kingdom)
C. Dunsby, Imperial College London (United Kingdom)
D. M. Owen, Imperial College London (United Kingdom)
N. P. Galletly, Imperial College London (United Kingdom)
U. Anand, Imperial College London (United Kingdom)
C. D. Benham, GlaxoSmithKline (United Kingdom)
A. Naylor, GlaxoSmithKline (United Kingdom)
G. W. Stamp, Imperial College London (United Kingdom)
P. Anand, Imperial College London (United Kingdom)
P. M. W. French, Imperial College London (United Kingdom)


Published in SPIE Proceedings Vol. 6433:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII
Israel Gannot, Editor(s)

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