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Proceedings Paper

Spectral domain optical coherence tomography for in-vivo three-dimensional retinal imaging of small animals
Author(s): Marco Ruggeri; Hassan Wehbe; Shuliang Jiao; Giovanni Gregori; Maria E. Jockovich; Abigail Hackam; Yuanli Duan; Carmen A. Puliafito
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Paper Abstract

The purpose of this study is to demonstrate the application of ultrahigh-resolution Spectral Domain Optical Coherence Tomography (SD-OCT) for non contact in vivo imaging of the retina of small animals and quantitative retinal information extraction using 3D segmentation of the OCT images. An ultrahigh-resolution SD-OCT system was specifically designed for in vivo retinal imaging of small animal. En face fundus image was constructed from the measured OCT data, which enables precise registration of the OCT images on the fundus. 3D segmentation algorithms were developed for the calculation of retinal thickness map. High quality OCT images of the retina of mice (B6/SJLF2 for normal retina, Rho-/- for photoreceptor degeneration and LHBETATAG for retinoblastoma) and rats (Wistar for normal retina) were acquired, where all the retinal layers can be clearly recognized. The calculated retinal thickness map makes successful quantitative comparison of the retinal thickness distribution between normal and degenerative mouse retina. The capabilities of the OCT system provide a valuable tool for longitudinal studies of small animal models of ocular diseases.

Paper Details

Date Published: 7 February 2007
PDF: 7 pages
Proc. SPIE 6429, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI, 64290S (7 February 2007); doi: 10.1117/12.700437
Show Author Affiliations
Marco Ruggeri, Univ. of Miami Miller School of Medicine (United States)
Hassan Wehbe, Univ. of Miami Miller School of Medicine (United States)
Shuliang Jiao, Univ. of Miami Miller School of Medicine (United States)
Giovanni Gregori, Univ. of Miami Miller School of Medicine (United States)
Maria E. Jockovich, Univ. of Miami Miller School of Medicine (United States)
Abigail Hackam, Univ. of Miami Miller School of Medicine (United States)
Yuanli Duan, Univ. of Miami Miller School of Medicine (United States)
Carmen A. Puliafito, Univ. of Miami Miller School of Medicine (United States)


Published in SPIE Proceedings Vol. 6429:
Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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