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Proceedings Paper

In vivo fluorescence enhanced optical tomography reconstruction of lung cancer of non immersed small animals
Author(s): L. Hervé; A. Koenig; A. Da Silva; M. Berger; J. Boutet; J. M. Dinten; P. Peltié; P. Rizo
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Paper Abstract

Fluorescence enhanced diffuse optical tomography (fDOT) is envisioned to be useful to collect functional information from small animal models. For oncology applications, cancer-targeted fluorescent markers can be used as a surrogate of the cancer activity. We are developing a continuous wave fDOT bench intended to be integrated in systems dedicated to whole body small animal fluorescence analyses. The focus is currently put on the reconstruction of non immersed small animals imaged by a CCD camera. The reconstruction stage already corrects the tissue heterogeneity artifacts through the computation of an optical heterogeneity map. We will show how this formalism coupled with the determination of the animal boundaries performed by a laser scanner, can be used to manage non contact acquisitions. The time of reconstruction for a 10 × 9 laser source positions, 45 × 40 detector elements and 14 × 11 × 14 mesh voxels is typically 10 minutes on a 3GHz PCs corresponding to the acquisition time allowing the two tasks to be performed in parallel. The system is validated on an in vivo experiment performed on three healthy nude mice and a mouse bearing a lung tumor at 10, 12 and 14 days after implantation allowing the follow up of the disease. The 3D fluorescence reconstructions of this mouse are presented and the total fluorescence amounts are compared.

Paper Details

Date Published: 13 February 2007
PDF: 8 pages
Proc. SPIE 6434, Optical Tomography and Spectroscopy of Tissue VII, 643410 (13 February 2007); doi: 10.1117/12.700337
Show Author Affiliations
L. Hervé, CEA-LETI, MINATEC (France)
A. Koenig, CEA-LETI, MINATEC (France)
A. Da Silva, CEA-LETI, MINATEC (France)
M. Berger, CEA-LETI, MINATEC (France)
J. Boutet, CEA-LETI, MINATEC (France)
J. M. Dinten, CEA-LETI, MINATEC (France)
P. Peltié, CEA-LETI, MINATEC (France)
P. Rizo, CEA-LETI, MINATEC (France)

Published in SPIE Proceedings Vol. 6434:
Optical Tomography and Spectroscopy of Tissue VII
Britton Chance; Robert R. Alfano; Bruce J. Tromberg; Mamoru Tamura; Eva Marie Sevick-Muraca, Editor(s)

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