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Proceedings Paper

CARS imaging with a new 532-nm synchronously pumped picosecond OPO
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Paper Abstract

A new, synchronously pumped picosecond OPO for CARS microscopy is presented. It is based on non-critically phasematched interaction in LBO pumped by a frequency-doubled modelocked Nd:Vanadat laser at 532 nm. Within the parametric process a tuneable pair of two different wavelengths in the NIR range is generated (Signal <680 ...990 nm, Idler 1150...>2450 nm). In this system they are extracted from the cavity at the same mirror and therefore propagating collinear at the same beam path. Due to the mechanism of their generation there is no jitter between Signal and Idler. Though the wavelengths are different the GVD is negligible for this picosecond pulse duration. As a result the two pulse trains are spatially and temporally perfectly matched. The pulses generated are close to transform limit with about 5-6 ps pulse duration, excellent beam quality (M2 < 1,1) and high pointing stability. The output power for Signal and Idler is about 1 W each @ 4 W pump power. The tuning mechanism is split into two parts - temperature tuning for rough variations and fast angular BRF tuning for the fine adjustment of the output wavelength. The perfect spatial and temporal overlap make the described OPO an ideal and nearly hands-free laser source for CARS microscopy with a tuneable energy difference 1,400 ... >10,000 cm-1. The absolute wavelength range is resulting in high penetration depth and low photo damage of the analyzed samples. Finally some CARS-images are presented and the latest results and methods for further sensitivity enhancements are shown.

Paper Details

Date Published: 10 February 2007
PDF: 8 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64420C (10 February 2007); doi: 10.1117/12.700072
Show Author Affiliations
Edlef Büttner, APE GmbH (Germany)
Silvia Carrasco, Harvard Univ. (United States)
Conor L. Evans, Harvard Univ. (United States)
Feruz S. Ganikhanov, Harvard Univ. (United States)
Johannes G. Herbst, APE GmbH (Germany)
Daniel Kopf, HighQ Laser Production GmbH (Austria)
Ingo Rimke, APE GmbH (Germany)
Sunney Xie, Harvard Univ. (United States)

Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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