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Proceedings Paper

Colloid-gold nanoparticle enhanced detection sensitivity of paired surface plasma waves biosensor
Author(s): Hsieh-Ting Wu; Yi-Ling Chen; Yen-Chen Huang; Wei-Chih Liu; Ming-Yaw Ng; Yin-Fong Chang; Bao-Yu Hsieh; Chien Chou
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Paper Abstract

Previous work with amplitude-sensitive paired surface plasma waves biosensor (PSPWB) demonstrated that the detection sensitivity of PSPWB is able to experimentally detect 0.001% sucrose-water solution and biomolecular interaction of 10pg/ml mouse IgG interacting with immobilized anti-mouse IgG successfully. Further development of the detection sensitivity of PSPWB has been conducted by using 20nm in diameter colloid gold nanoparticles conjugated with target molecules that can result in a higher mass coverage and a larger resonant angle change of plasmon resonance, thereby its detection sensitivity is further enhanced significantly. Bare gold nanoparticles, which is randomly suspended in solution, is adopted to differentiate biospecific binding induced further signal enhancement. Experimentally, the sensitivity at 330fg/ml of Au-nanoparticle conjugated protein A (PA-Au) interacting with mouse IgG which is immobilized on a CM5 sensor chip was detected successfully. By this arrangement, 6-fold signal amplification is demonstrated compared with the same concentration of PA without conjugated gold nanoparticles.

Paper Details

Date Published: 12 February 2007
PDF: 7 pages
Proc. SPIE 6447, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV, 64470S (12 February 2007); doi: 10.1117/12.700009
Show Author Affiliations
Hsieh-Ting Wu, National Yang Ming Univ. (Taiwan)
Yi-Ling Chen, National Yang Ming Univ. (Taiwan)
Yen-Chen Huang, National Yang Ming Univ. (Taiwan)
Wei-Chih Liu, National Taiwan Normal Univ. (Taiwan)
Ming-Yaw Ng, National Taiwan Normal Univ. (Taiwan)
Yin-Fong Chang, National Central Univ. (Taiwan)
Bao-Yu Hsieh, National Yang Ming Univ. (Taiwan)
Chien Chou, National Yang Ming Univ. (Taiwan)
National Central Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6447:
Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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