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Proceedings Paper

Raman versus CARS microscopy: when one is better than the other
Author(s): G. I. Petrov; R. Arora; A. Saha; R. D. Heathcote; S. Ravula; I. Brener; V. V. Yakovlev
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Paper Abstract

Since the first introduction of Raman microscope in 1973, optical and laser technology has made a tremendous step forward. However, despite of the obvious advantages of being a very informative and nondestructive method of studying biological samples, spontaneous Raman scattering suffers from a series of limitations such as a fluorescent background and a low signal level. Nonlinear Raman spectroscopy and, in particular, spectroscopy of coherent anti-Stokes Raman scattering (CARS) can resolve most of the problems associated with conventional Raman spectroscopy. In this report, the most critical issues of the CARS microspectroscopy setup design are reviewed and several exciting potential applications of the broadband CARS microspectroscopy, where the CARS microscopy has an advantage with respect to Raman microscopy, are outlined.

Paper Details

Date Published: 10 February 2007
PDF: 8 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 644209 (10 February 2007); doi: 10.1117/12.700007
Show Author Affiliations
G. I. Petrov, Univ. of Wisconsin, Milwaukee (United States)
R. Arora, Univ. of Wisconsin, Milwaukee (United States)
A. Saha, Univ. of Wisconsin, Milwaukee (United States)
R. D. Heathcote, Univ. of Wisconsin, Milwaukee (United States)
S. Ravula, Sandia National Labs. (United States)
I. Brener, Sandia National Labs. (United States)
V. V. Yakovlev, Univ. of Wisconsin, Milwaukee (United States)


Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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