Share Email Print
cover

Proceedings Paper

Intravital multiphoton microscopy for imaging hepatobiliary function
Author(s): Wen Lo; Yuan Liu; Hsiao-Ching Chen; Shu-Mei Yang; Tzu-Lin Sun; Ling-Ling Chiou; Guan-Tarn Huang; Chen-Yuan Dong; Hsuan-Shu Lee
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In this work, intravital multiphoton microscopy is applied to the imaging of liver metabolism with the least invasiveness. We observed intravital dynamics of the uptake, processing and excretion of the organic anion species, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary system. This is achieved by the use of multiphoton microscopy and an in vivo hepatic imaging chamber which allows us to image the dynamics of hepatic metabolism. Multiphoton images revealed that the hepatic processing of 6-CFDA is completed within approximately 50 minutes. The images reveal the liver metabolism of the uptake and processing of 6-CFDA from the hepatocytes, and the subsequent excretion into bile canaliculi. Our results suggest that this approach is a promising technique for investigating intravital hepatic physiology, diseases, and metabolism.

Paper Details

Date Published: 10 February 2007
PDF: 8 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421R (10 February 2007); doi: 10.1117/12.699896
Show Author Affiliations
Wen Lo, National Taiwan Univ. (Taiwan)
Yuan Liu, National Taiwan Univ. (Taiwan)
Hsiao-Ching Chen, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Shu-Mei Yang, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Tzu-Lin Sun, National Taiwan Univ. (Taiwan)
Ling-Ling Chiou, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Guan-Tarn Huang, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Hsuan-Shu Lee, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top