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Proceedings Paper

Structural analysis of blended materials using multiphoton autofluorescence and second harmonic generation microscopy
Author(s): Hsin-Yuan Tan; Ming-Guo Lin; Sung-Jan Lin; Wen-Chu Hsiao; Liang-Kung Wen; Wei-Liang Chen; Tai-Horng Young; Chen-Yuan Dong
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Paper Abstract

The purpose of this work is to investigate the microstructure of blended materials using non-invasive, optical imaging modality. Multiphoton autofluorescence and second harmonic generation signals will be used for characterizing and quantifying individual non-linear optical properties of each polymer in pure polymeric thin films. In addition, reflected confocal signals will used to outline the interface of refractive index mismatch. And the phase separation phenomenon of immiscible blended membranes composed of different ratio of nylon and chitosan are analyzed and differentiated using the non-invasive optical information including autofluerescence, second harmonic generation, and reflected signals. We therefore propose the potentiality of using multiphoton autofluorescence and second harmonic generation microscopy complemented with reflected confocal microscopy for studying the synthetic blended polymeric scaffolds and also, in the future, the dynamic, in vivo, cell-matrix interaction in the field of tissue engineering.

Paper Details

Date Published: 7 February 2007
PDF: 8 pages
Proc. SPIE 6439, Optics in Tissue Engineering and Regenerative Medicine, 643904 (7 February 2007); doi: 10.1117/12.699894
Show Author Affiliations
Hsin-Yuan Tan, College of Medicine and College of Engineering (Taiwan)
Chang Gung Memorial Hospital and Chang Gung Univ. (Taiwan)
Ming-Guo Lin, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan University and National Taiwan Univ. Hospital (Taiwan)
Wen-Chu Hsiao, College of Medicine and College of Engineering (Taiwan)
Liang-Kung Wen, National Taiwan Univ. (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Tai-Horng Young, College of Medicine and College of Engineering (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6439:
Optics in Tissue Engineering and Regenerative Medicine
Sean J. Kirkpatrick; Ruikang K. Wang, Editor(s)

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