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Proceedings Paper

A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy
Author(s): Kyujung Kim; Donghyun Kim; Eun-jin Cho; Yong-min Huh
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Paper Abstract

For investigatoin of intracellular protein interactions, quantum dots are widely used for fluorescent live cell imaging such as total internal reflection fluorescence (TIRF) microscopy and confocal microscopy. In this paper, we performed a quantitative analysis based on fluorescent intensity. For the measurement, A431 cell lines are imaged live with quantum dots using TIRF microscopy. The distribution of quantum dots is affected by a TATHA2 peptide sequence in live cells. This paper also presents the location change of quantum dots due to a nuclear localization signal in A431 cell lines. Confocal microscopy was used to confirm the relation with fluorescent intensity and quantum dot concentration in live cells.

Paper Details

Date Published: 14 February 2007
PDF: 7 pages
Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 64490K (14 February 2007); doi: 10.1117/12.699874
Show Author Affiliations
Kyujung Kim, Yonsei Univ. (South Korea)
Donghyun Kim, Yonsei Univ. (South Korea)
Eun-jin Cho, Yonsei Univ. (South Korea)
College of Medicine, Yonsei Univ. (South Korea)
Yong-min Huh, Yonsei Univ. (South Korea)
College of Medicine, Yonsei Univ. (South Korea)


Published in SPIE Proceedings Vol. 6449:
Genetically Engineered and Optical Probes for Biomedical Applications IV
Samuel Achilefu; Alexander Pavlovich Savitsky; Rebekka M. Wachter; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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