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Proceedings Paper

TIRET microscopy: monitoring protein (amyloid precursor protein and beta-secretase) interaction on the surface of living cells
Author(s): Christine von Arnim; Michael Wagner; Petra Weber; Herbert Schneckenburger
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Paper Abstract

Total internal reflection fluorescence microscopy (TIRFM) and non-radiative energy transfer (FRET) measurements have been combined in order to examine co-localization of the amyloid precursor protein (APP) and the ?-site APPcleaving enzyme (BACE) in human glioblastoma cells. So far, these proteins have been co-localized within whole cells (depending on the intracellular amount of cholesterol) and in some cases also within their plasma membranes. This supports the present hypothesis of localization within lipid domains on the cell surface and co-internalization via endocytosis.

Paper Details

Date Published: 19 February 2007
PDF: 8 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410D (19 February 2007); doi: 10.1117/12.699856
Show Author Affiliations
Christine von Arnim, Univ. Ulm (Germany)
Michael Wagner, Institut für Angew, Hochschule Aalen (Germany)
Petra Weber, Institut für Angew, Hochschule Aalen (Germany)
Herbert Schneckenburger, Institut für Angew, Hochschule Aalen (Germany)


Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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