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Proceedings Paper

Axially resolved cell imaging by intensity modulated total internal reflection fluorescence microscopy (IM-TIRFM)
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Paper Abstract

Intensity modulation was introduced to total internal reflection fluorescence microscopy (TIRFM). This permits to obtain information about the axial distribution of fluorescent dyes in close vicinity to the surface of adherent cells and allows the application of (commercial) objective based TIRFM systems at a fixed angle of illumination. However, this new method seems to be less suitable for quantitative measurements of cell-substrate topology as compared to variableangle prism based TIRFM.

Paper Details

Date Published: 19 February 2007
PDF: 6 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411E (19 February 2007); doi: 10.1117/12.699826
Show Author Affiliations
Herbert Schneckenburger, Institut für Angewandte Forschung, Hochschule Aalen (Germany)
Institut für Lasertechnologien in der Medizin und Messtechnik, Univ. Ulm (Germany)
Harald Baumann, Institut für Angewandte Forschung, Hochschule Aalen (Germany)
Michael Wagner, Institut für Angewandte Forschung, Hochschule Aalen (Germany)
Wolfgang S. L. Strauss, Institut für Lasertechnologien in der Medizin und Messtechnik, Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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