Share Email Print
cover

Proceedings Paper

Measurement of relative phase distribution of onion epidermal cells by using the polarization microscope
Author(s): In Hee Shin; Ji Yong Lee; Seungrag Lee; Dong Ju Lee; Dug Young Kim
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Bio-cells and tissues have intrinsic polarization characteristics, which are changed by external stimulus and internal metamorphosis in cells and tissues and some of the bio-cells and tissues have intrinsic birefringence characteristics, which are also changed by external stimulus and internal metamorphosis in cells and tissues. In this paper, we have developed the polarization microscope for measurement of relative phase which results from birefringence characteristics of materials with improved linear polarizing method and have measured relative phase distribution of onion epidermal cells. From the measurement of the relative phase distribution of onion epidermal cells, decrease of relative phase distribution of onion epidermal cells was investigated as the elapse of time. In decrease of relative phase distribution, relative phase of cell membrane in onion epidermal cells decreased radically as compared with that of cytoplasm because decline of function in cell membrane that takes charge of matter transfer in onion epidermal cells has occurred.

Paper Details

Date Published: 14 February 2007
PDF: 7 pages
Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 644317 (14 February 2007); doi: 10.1117/12.699785
Show Author Affiliations
In Hee Shin, Gwangju Institute of Science and Technology (South Korea)
Ji Yong Lee, Gwangju Institute of Science and Technology (South Korea)
Seungrag Lee, Gwangju Institute of Science and Technology (South Korea)
Dong Ju Lee, Gwangju Institute of Science and Technology (South Korea)
Dug Young Kim, Gwangju Institute of Science and Technology (South Korea)


Published in SPIE Proceedings Vol. 6443:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

© SPIE. Terms of Use
Back to Top