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Proceedings Paper

In-vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual-UV excitation
Author(s): Rajesh N. Raman; Christopher D. Pivetti; Dennis L. Matthews; Christoph Troppmann; Stavros G. Demos
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Paper Abstract

We explore an optical spectroscopy approach to monitor the progression of ischemia and reperfusion in situ using a rat model. The system utilizes the sensitivity of NADH emission to changes in cell metabolism during ischemia and reperfusion. In addition, the emission from tryptophan is employed as a normalization against changes in other optical properties of the tissue. Ischemia was induced in one kidney followed by at least 60 minutes of reperfusion. During both phases, autofluorescence images of the exposed surfaces of both the ischemic kidney and the normal (control) kidney were acquired and the respective average emission intensities were determined. Preliminary results indicate that the kinetics of the ratio of the emissions under these two excitations is related to the injury time.

Paper Details

Date Published: 19 February 2007
PDF: 8 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410J (19 February 2007); doi: 10.1117/12.699744
Show Author Affiliations
Rajesh N. Raman, Univ. of California, Davis (United States)
Christopher D. Pivetti, Univ. of California, Davis Medical Ctr. (United States)
Dennis L. Matthews, Univ. of California, Davis (United States)
Christoph Troppmann, Univ. of California, Davis Medical Ctr. (United States)
Stavros G. Demos, Lawrence Livermore National Lab. (United States)
Univ. of California, Davis Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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