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Proceedings Paper

Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography: laser induced fluorescence
Author(s): N. Sujatha; Lavanya Rai; Pratap Kumar; B. R. Krishnanand; K. K. Mahato; Sajan D. George; V. B. Kartha; Santhosh C
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Paper Abstract

HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

Paper Details

Date Published: 19 February 2007
PDF: 8 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410B (19 February 2007); doi: 10.1117/12.699711
Show Author Affiliations
N. Sujatha, Ctr. for Laser Spectroscopy, MAHE Life Sciences Ctr. (India)
Lavanya Rai, Kasturba Medical College, MAHE, Manipal (India)
Pratap Kumar, Kasturba Medical College, MAHE, Manipal (India)
B. R. Krishnanand, Kasturba Medical College, MAHE, Manipal (India)
K. K. Mahato, Ctr. for Laser Spectroscopy, MAHE Life Sciences Ctr. (India)
Sajan D. George, Ctr. for Laser Spectroscopy, MAHE Life Sciences Ctr. (India)
V. B. Kartha, Ctr. for Laser Spectroscopy, MAHE Life Sciences Ctr. (India)
Santhosh C, Ctr. for Laser Spectroscopy, MAHE Life Sciences Ctr. (India)


Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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