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Proceedings Paper

In-vitro visualization of corneal wound healing in an organ culture model using multiphoton autofluorescence and second harmonic generation microscopy
Author(s): Wen Lo; Yuh-Ling Chang; Yen Sun; Sung-Jan Lin; Shiou-Hwa Jee; Hsin-Yuan Tan; Chen-Yuan Dong
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Paper Abstract

The aim of this work is to image the wound healing process of cornea in an in vitro organ culture model with noninvasive multiphoton imaging modality. Autofluorescence and second harmonic generation (SHG) were respectively used to monitor the alterations of cellular and collagenous components during wound healing processes. Within additional developments, this approach may be applied to in vivo visualization of corneal structural destruction and the subsequent regeneration.

Paper Details

Date Published: 26 March 2007
PDF: 8 pages
Proc. SPIE 6426, Ophthalmic Technologies XVII, 642617 (26 March 2007); doi: 10.1117/12.699709
Show Author Affiliations
Wen Lo, National Taiwan Univ. (Taiwan)
Yuh-Ling Chang, National Taiwan Univ. (Taiwan)
Yen Sun, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. and National Taiwan Univ. Hospital (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. and National Taiwan Univ. Hospital (Taiwan)
Hsin-Yuan Tan, Institute of Medical Engineering (Taiwan)
Chang Gung Univ. (Taiwan)
Chang Gung Memorial Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6426:
Ophthalmic Technologies XVII
Bruce E. Stuck; Fabrice Manns; Per G. Söderberg; Michael Belkin M.D.; Arthur Ho, Editor(s)

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