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Proceedings Paper

Coherent anti-Stokes Raman scattering microscope with a high-signal-to-noise ratio, high stability, and high-speed imaging for live cell observation
Author(s): Shinichi Hayashi; Shinichi Takimoto; Takeshi Hashimoto
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Paper Abstract

Coherent anti-Stokes Raman scattering (CARS) microscopy, which can produce images of specific molecules without staining, has attracted the attention of researchers, as it matches the need for molecular imaging and pathway analysis of live cells. In particular, there have been an increasing number of CARS experimental results regarding lipids in live cells, which cannot be fluorescently tagged while keeping the cells alive. One of the important applications of lipid research is for the metabolic syndrome. Since the metabolic syndrome is said to be related to the lipids in lipocytes, blood, arterial vessels, and so on, the CARS technique is expected to find application in this field. However, CARS microscopy requires a pair of picosecond laser pulses, which overlap both temporally and spatially. This makes the optical adjustments of a CARS microscope challenging. The authors developed a CARS unit that includes optics for easy and stable adjustment of the overlap of these laser pulses. Adding the CARS unit to a laser scanning microscope provides CARS images of a high signal-to-noise ratio, with an acquisition rate as high as 2 microseconds per pixel. Thus, images of fast-moving lipid droplets in Hela cells were obtained.

Paper Details

Date Published: 10 February 2007
PDF: 9 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 644208 (10 February 2007); doi: 10.1117/12.699639
Show Author Affiliations
Shinichi Hayashi, Olympus Corp. (Japan)
Shinichi Takimoto, Olympus Corp. (Japan)
Takeshi Hashimoto, Olympus Corp. (Japan)

Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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