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Proceedings Paper

Time-resolved evanescent wave-induced fluorescence studies of macromolecular adsorption
Author(s): Trevor A. Smith; Colin A. Scholes; Michelle L. Gee
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Paper Abstract

Evanescent Wave (EW) fluorescence spectroscopic techniques are exploited to obtain information about the conformational state of macromolecules within close proximity of a solid/liquid interfacial region. Specifically, timeresolved evanescent wave-induced fluorescence techniques have been applied to the study of the adsorption of polymers and biomolecules to silica surfaces. We have extended these EW measurements using polarized excitation and emission detection to probe molecular motion and conformational change in the microenvironment of the interfacial region. We report on the observation of complex time-dependent fluorescence anisotropy data and the interpretation of these data in terms of in- and out-of-plane rotational motion. The macromolecular-interfacial systems investigated by this evanescent wave approach included polymer film dynamics and adsorbed protein rearrangement upon adsorption.

Paper Details

Date Published: 9 February 2007
PDF: 9 pages
Proc. SPIE 6483, Complex Light and Optical Forces, 64830C (9 February 2007); doi: 10.1117/12.699637
Show Author Affiliations
Trevor A. Smith, Univ. of Melbourne (Australia)
Colin A. Scholes, Univ. of Melbourne (Australia)
Michelle L. Gee, Univ. of Melbourne (Australia)

Published in SPIE Proceedings Vol. 6483:
Complex Light and Optical Forces
David L. Andrews; Enrique J. Galvez; Gerard Nienhuis, Editor(s)

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