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Proceedings Paper

Real-time single cell analysis of Bid cleavage and translocation in cisplatin-induced apoptosis
Author(s): Lei Liu; Da Xing; Yihui Pei; Wei R. Chen
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Paper Abstract

Cancer cell apoptosis can be induced by cisplatin, an efficient anticancer agent. However, its mechanism is not fully understood. Bcl-2 homology domain (BH) 3-only proteins couple stress signals to mitochondrial apoptotic pathways. Calpain-mediated cleavage of the BH3-only protein Bid into a 14 kD truncated protein (tBid) has been implicated in cisplatin-induced apoptotic pathway. We utilized a recombinant fluorescence resonance energy transfer (FRET) Bid probe to determine the kinetics of Bid cleavage during cisplatin-induced apoptosis in ASTC-a-1 cells. The cells were also co-transfected with Bid-CFP and DsRed-Mit to dynamically detect tBid translocation. Cells showed a cleavage of the Bid-FRET probe occurring at about 4-5 h after treated with 20 µM cisplatin. Cleavage of the Bid-FRET probe coincided with a translocation of tBid from the cytosolic to the mitochondria, and the translocation lasted about 1.5 h. Using real-time single-cell analysis, we first observed the kinetics of Bid cleavage and translocation to mitochondria in living cells during cisplatin-induced apoptosis.

Paper Details

Date Published: 13 February 2007
PDF: 9 pages
Proc. SPIE 6438, Biophotonics and Immune Responses II, 64380G (13 February 2007); doi: 10.1117/12.699634
Show Author Affiliations
Lei Liu, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Yihui Pei, South China Normal Univ. (China)
Wei R. Chen, South China Normal Univ. (China)
Univ. of Central Oklahoma (United States)


Published in SPIE Proceedings Vol. 6438:
Biophotonics and Immune Responses II
Wei R. Chen, Editor(s)

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