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Proceedings Paper

An optical approach for non-invasive blood clot testing
Author(s): Vyacheslav Kalchenko; Alexander Brill; Ilya Fine; Alon Harmelin
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Paper Abstract

Physiological blood coagulation is an essential biological process. Current tests for plasma coagulation (clotting) need to be performed ex vivo and require fresh blood sampling for every test. A recently published work describes a new, noninvasive, in vivo approach to assess blood coagulation status during mechanical occlusion1. For this purpose, we have tested this approach and applied a controlled laser beam to blood micro-vessels of the mouse ear during mechanical occlusion. Standard setup for intravital transillumination videomicroscopy and laser based imaging techniques were used for monitoring the blood clotting process. Temporal mechanical occlusion of blood vessels in the observed area was applied to ensure blood flow cessation. Subsequently, laser irradiation was used to induce vascular micro-injury. Changes in the vessel wall, as well as in the pattern of blood flow, predispose the area to vascular thrombosis, according to the paradigm of Virchow's triad. In our experiments, two elements of Virchow's triad were used to induce the process of clotting in vivo, and to assess it optically. We identified several parameters that can serve as markers of the blood clotting process in vivo. These include changes in light absorption in the area of illumination, as well as changes in the pattern of the red blood cells' micro-movement in the vessels where blood flow is completely arrested. Thus, our results indicate that blood coagulation status can be characterized by non-invasive, in vivo methodologies.

Paper Details

Date Published: 14 February 2007
PDF: 8 pages
Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 644508 (14 February 2007); doi: 10.1117/12.699491
Show Author Affiliations
Vyacheslav Kalchenko, Weizmann Institute of Science (Israel)
Alexander Brill, Hadassah Hebrew Univ. Medical Center (Israel)
Ilya Fine, ELFI-Tech Ltd. (Israel)
Alon Harmelin, Weizmann Institute of Science (Israel)


Published in SPIE Proceedings Vol. 6445:
Optical Diagnostics and Sensing VII
Gerard L. Coté; Alexander V. Priezzhev, Editor(s)

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