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Proceedings Paper

Mie scattering model for dual-axes confocal architecture
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Paper Abstract

Tissue scattering has a significant effect on the image resolution and light collection efficiency in confocal microscopy. The dual-axes (DA) confocal architecture has many advantages including high axial resolution with low numerical aperture lenses and long working distance for use in vivo as a microendoscope. In addition, less scattered light along the illumination path may be collected and introduced as noise. In this paper, we use Monte Carlo tissue scattering simulations to compare the dual-axes and conventional single-axis (SA) configurations. Simulation results show that the axial response for the dual axes configuration varies with pinhole size and optical thickness of scattering media in a way that differs from the single axis architecture. The DA configuration is able to filter out efficiently multiply-scattered photons and out-of-focus light, thus allowing imaging with greater tissue penetration depths to provide vertical crosssectional images, which has significant implications for in vivo imaging.

Paper Details

Date Published: 14 February 2007
PDF: 8 pages
Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430R (14 February 2007); doi: 10.1117/12.699437
Show Author Affiliations
Larry K. Wong, Stanford Univ. School of Medicine (United States)
Michael J Mandella, Gintzon Lab., Stanford Univ. (United States)
Paul Holcomb, Breault Research Organization, Inc. (United States)
Gordon S. Kino, Gintzon Lab., Stanford Univ. (United States)
Thomas D. Wang, Stanford Univ. School of Medicine (United States)


Published in SPIE Proceedings Vol. 6443:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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