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Proceedings Paper

Quantum dot-DNA bioconjugates for fluorescence-resonance-energy-transfer-based biosensing
Author(s): Igor L. Medintz; Lorenzo Berti; Thomas Pons; Hedi Mattoussi
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Paper Abstract

Semiconductor quantum dots (QDs) have unique photophysical properties which make them excellent fluorescence resonance energy transfer donors. However, lack of facile methods for conjugating biomolecules such as DNA, proteins and peptides to QDs have limited their applications. In this report, we describe a general procedure for the preparation of a synthetic peptide that can be covalently attached to DNA segments and used to facilitate the self-assembly of the modified DNA onto water soluble QDs. To characterize this conjugation strategy, dye-labeled DNA is first reacted with the synthetic peptide and the resulting peptide-DNA then self-assembled onto QDs. QD attachment is verified by monitoring resonance energy transfer efficiency from the QD donor to the dye-labeled DNA acceptor. QD-DNA bioconjugates assembled using this method may find applications as molecular beacons and hybridization probes.

Paper Details

Date Published: 27 February 2007
PDF: 7 pages
Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480G (27 February 2007); doi: 10.1117/12.699384
Show Author Affiliations
Igor L. Medintz, Naval Research Lab. (United States)
Lorenzo Berti, S3-INFM-CNR (Italy)
Thomas Pons, Naval Research Lab. (United States)
Hedi Mattoussi, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 6448:
Colloidal Quantum Dots for Biomedical Applications II
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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