Share Email Print
cover

Proceedings Paper

Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography
Author(s): Lida P. Hariri; Ziping Qiu; Alexandre R. Tumlinson; David G. Besselsen; Eugene W. Gerner; Natalia Ignatenko; Boris Považay; Boris Hermann; Harald Sattmann; James McNally; Angelika Unterhuber; Wolfgang Drexler; Jennifer K. Barton
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Purpose: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be commissioned for small diameter endoscopic applications for imaging mouse models of colorectal cancer. In this study, we utilized ultrahigh resolution OCT (UHR OCT) to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC, monitor the progression of neoplastic transformations, and determine if OCT is capable of identifying early disease. Experimental Design: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22, and 26 weeks post AOM treatment) using a prototype 2.0 mm diameter UHR OCT endoscope-based system that achieved resolutions of 3.2 um axial and 4.4 um lateral. Histological samples were obtained at the final imaging timepoint serving as the gold standard. Results: Gross and histological assessment of the excised colonic tissue revealed at least one tumor in all 13 AOM treated mice, with most mice developing multiple tumors. In the corresponding OCT images, a progression from healthy thin mucosa to adenoma appearing as large, structurally disorganized masses was visualized over the imaging time points correlating to the locations of the grossly visualized tumors. Conclusions: This study indicates that UHR OCT enables accurate identification of disease and non-destructive visualization of CRC progression in the lower colon of mice.

Paper Details

Date Published: 13 February 2007
PDF: 10 pages
Proc. SPIE 6432, Endoscopic Microscopy II, 643208 (13 February 2007); doi: 10.1117/12.699249
Show Author Affiliations
Lida P. Hariri, The Univ. of Arizona (United States)
Ziping Qiu, Medical Univ. of Vienna (Austria)
Alexandre R. Tumlinson, The Univ. of Arizona (United States)
David G. Besselsen, The Univ. of Arizona (United States)
Eugene W. Gerner, The Univ. of Arizona (United States)
Natalia Ignatenko, The Univ. of Arizona (United States)
Boris Považay, Cardiff Univ. (United Kingdom)
Medical Univ. of Vienna (Austria)
Boris Hermann, Medical Univ. of Vienna (Austria)
Cardiff Univ. (United Kingdom)
Harald Sattmann, Medical Univ. of Vienna (Austria)
Cardiff Univ. (United Kingdom)
James McNally, College of Optical Sciences, The Univ. of Arizona (United States)
Angelika Unterhuber, Medical Univ. of Vienna (Austria)
Cardiff Univ. (United Kingdom)
Wolfgang Drexler, Medical Univ. of Vienna (Austria)
Cardiff Univ. (United Kingdom)
Jennifer K. Barton, The Univ. of Arizona (United States)
College of Optical Sciences, The Univ. of Arizona (United States)


Published in SPIE Proceedings Vol. 6432:
Endoscopic Microscopy II
Guillermo J. Tearney; Thomas D. Wang, Editor(s)

© SPIE. Terms of Use
Back to Top