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Proceedings Paper

Interleaved dual-wavelength multiphoton imaging system for heterologous FRET and versatile fluorescent protein excitation
Author(s): M. Anna Zal; Michelle Nelson; Tomasz Zal
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Paper Abstract

Broad two-photon cross sections of fluorescent proteins allow excitation with a single wavelength of a tunable femtosecond pulsed laser but the brightness is sub-optimal and the cross-talk prevents sensitized emission FRET imaging in heterologous systems. We present a novel arrangement of a resonant scanning microscope capable of fast interline dual wavelength femtosecond excitation of pairs of fluorophores. This allows optimal and selective excitation of mCerulean and mCitrine as well as FRET imaging using the principles of sensitized emission 3-cube imaging. Performance of the system in thin and thick specimens is discussed.

Paper Details

Date Published: 16 February 2007
PDF: 7 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421Q (16 February 2007); doi: 10.1117/12.699242
Show Author Affiliations
M. Anna Zal, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Michelle Nelson, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Tomasz Zal, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)


Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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