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Proceedings Paper

Microwave-accelerated plasmonics: application to ultrafast and ultrasensitive clinical assays
Author(s): Kadir Aslan; Michael J. R. Previte; Yongxia Zhang; Chris D. Geddes
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Paper Abstract

In recent years our laboratory has described the favorable effects of fluorophores in close proximity to metallic nanostructures (1-6). These include, increased system quantum yields (increased detectability) and much improved fluorophore photostabilities. These effects have led to many applications of metal-enhanced fluorescence (MEF) including, improved DNA detection (7, 8), enhanced ratiometric sensing (5), metal-enhanced phosphorescence (9) and chemiluminescence signatures (10), as well as to the development of nano-rod (6), triangular nano-plate (4) and modified plastic surfaces (1, 3) for their multifarious applications. In all of our applications of MEF to date, we have been able to significantly optically amplify luminescence based signatures, but have been unable to modify the rates of the respective biochemical reactions being either studied or utilized, as these are dependent on the usual solution parameters of temperature, viscosity and their bioaffinity etc. However, our laboratory has recently shown that low power microwaves, when applied to the metallic nanostructures which are suitable for MEF, are preferentially heated, rapidly accelerating local biochemical reactions (11). Subsequently, ultra-fast and ultra-sensitive assays can be realized. We have recently termed the amalgamation of both MEF with microwave heating as "Microwave-Accelerated Metal-Enhanced Fluorescence (MAMEF)." In this conference proceeding, we summarize our MAMEF work on ultra-fast and sensitive myoglobin detection for rapid cardiac risk assessment and DNA detection for bioterrorism applications. In addition we present two new platform technologies, namely, Microwave-Accelerated Surface Plasmon-Coupled Directional Luminescence (MA-SPCL) for ultra fast assays using clinical samples and a Microwave-Accelerated Aggregation Assay (MA-AA) technology, for ultra fast solutionbased nanoparticle aggregation assays.

Paper Details

Date Published: 27 February 2007
PDF: 12 pages
Proc. SPIE 6450, Plasmonics in Biology and Medicine IV, 645007 (27 February 2007); doi: 10.1117/12.699159
Show Author Affiliations
Kadir Aslan, Univ. of Maryland Biotechnology Institute (United States)
Michael J. R. Previte, Univ. of Maryland Biotechnology Institute (United States)
Yongxia Zhang, Univ. of Maryland Biotechnology Institute (United States)
Chris D. Geddes, Univ. of Maryland Biotechnology Institute (United States)

Published in SPIE Proceedings Vol. 6450:
Plasmonics in Biology and Medicine IV
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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