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Proceedings Paper

Sensitivity enhancement of fiber optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles
Author(s): Ying-Feng Chang; Bao-Yu Hsieh; Hsiang-Yin Chen; Ming-Yaw Ng; Wei-Chih Liu; Yung-Chang Chung; Hsieh-Ting Wu; Chien Chou
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Paper Abstract

We proposed and developed a novel fiber-optic biosensor based on localized surface plasmon coupled emission (LSPCE) which consists of sandwich format of immuno-complex. It is immobilized on the surface of optical fiber where is a fluorescence probe produced by mixing Cy5 labeled antibody and protein A conjugated gold nanoparticles (Au-PA). The fluorophores are excited by localized surface plasmon (LSP) on gold nanoparticle (GNP) surface where the evanescent field is applied near the core surface of unclad optical fiber. Meanwhile, the fluorescence signal is detected by a photomultiplier tube being set beside the unclad optical fiber with high collection efficiency. In the experiment, this novel LSPCE biosensor demonstrates the minimum detectable concentration of mouse immunoglobulin G (IgG) at 1pg/ml (7fM) in the biomolecular interaction with anti-mouse IgG. From the experimental result, it verifies that LSPCE biosensor is a very high sensitive biosensor which is capable of measuring biomolecular interaction at very low concentration.

Paper Details

Date Published: 12 February 2007
PDF: 9 pages
Proc. SPIE 6447, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV, 64470R (12 February 2007); doi: 10.1117/12.699113
Show Author Affiliations
Ying-Feng Chang, National Central Univ. (Taiwan)
Bao-Yu Hsieh, National Yang Ming Univ. (Taiwan)
Hsiang-Yin Chen, National Yang Ming Univ. (Taiwan)
Ming-Yaw Ng, National Taiwan Normal Univ. (Taiwan)
Wei-Chih Liu, National Taiwan Normal Univ. (Taiwan)
Yung-Chang Chung, National Yang Ming Univ. (Taiwan)
Hsieh-Ting Wu, National Yang Ming Univ. (Taiwan)
Chien Chou, National Central Univ. (Taiwan)
National Yang Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6447:
Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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