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Proceedings Paper

A new optical method for characterizing single molecule interactions based on dark field microscopy
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Paper Abstract

Single-molecule techniques continue to gain in popularity in research disciplines such as the study of intermolecular interactions. These techniques provide information that otherwise would be lost by using bulk measurements that deal with a large number of molecules. We describe in this report the motion of tethered DNA molecules that have been tagged with gold nanobeads and observed under dark field microscopy to study single molecular interactions (SMI). We further report on the derivation and use of several physical parameters and how these parameters change under differing experimental conditions.

Paper Details

Date Published: 10 February 2007
PDF: 8 pages
Proc. SPIE 6444, Ultrasensitive and Single-Molecule Detection Technologies II, 644403 (10 February 2007); doi: 10.1117/12.699040
Show Author Affiliations
Heidelinde R. C. Dietrich, Delft Univ. of Technology (Netherlands)
Bart J. Vermolen, Delft Univ. of Technology (Netherlands)
Bernd Rieger, Delft Univ. of Technology (Netherlands)
Ian T. Young, Delft Univ. of Technology (Netherlands)
Yuval Garini, Delft Univ. of Technology (Netherlands)

Published in SPIE Proceedings Vol. 6444:
Ultrasensitive and Single-Molecule Detection Technologies II
Jorg Enderlein; Zygmunt K. Gryczynski, Editor(s)

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