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Proceedings Paper

A fluorescence biochip with a plasmon active surface
Author(s): Daniel R. Matthews; Huw D. Summers; Kerenza Njoh; Sally Chappell; Rachel Errington; Paul Smith; Iestyn Pope; Paul Barber; Boris Vojnovic; Simon Ameer-Beg
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Paper Abstract

We present details of the development of a optical biochip, with integrated on-chip laser excitation, for fluorescence intensity cell based assays. The biochip incorporates an "active surface" for the control and manipulation of fluorescent species placed directly on the device. The active elements of the biochip are one-dimensional periodic sub-wavelength corrugations fabricated on a thin gold film. We have made fluorescence intensity measurements of both an organic dye (Cy5), and immobilized and fluorescently labeled (with 705 nm emitting quantum dots), mammalian tumor cells in contact with the active surface. Here we show that the presence of the periodic grating can be used to control both the excitation and fluorescence generation process itself. We demonstrate that the gratings convert evanescent surface optical modes into well-defined beams of radiation in the far-field and at the surface of the device this produces highly contrasting regions of fluorescence excitation providing regions of high spatial selectivity.

Paper Details

Date Published: 14 February 2007
PDF: 8 pages
Proc. SPIE 6450, Plasmonics in Biology and Medicine IV, 645006 (14 February 2007); doi: 10.1117/12.698948
Show Author Affiliations
Daniel R. Matthews, Cardiff Univ. (United Kingdom)
Huw D. Summers, Cardiff Univ. (United Kingdom)
Kerenza Njoh, Cardiff Univ. (United Kingdom)
Sally Chappell, Cardiff Univ. (United Kingdom)
Rachel Errington, Cardiff Univ. (United Kingdom)
Paul Smith, Cardiff Univ. (United Kingdom)
Iestyn Pope, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)
Paul Barber, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)
Boris Vojnovic, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)
Simon Ameer-Beg, King's College Hospital (United Kingdom)

Published in SPIE Proceedings Vol. 6450:
Plasmonics in Biology and Medicine IV
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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