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Proceedings Paper

Nanofluidic channel based biosensor using surface enhanced raman spectroscopy (SERS)
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Paper Abstract

The Raman scattering signature of molecules has been demonstrated to be greatly enhanced, on the order of 106-1012 times, on roughened metal surfaces and clustered structures such as aggregated colloidal gold. Here we describe a method that improves reproducibility and sensitivity of the substrate for surface enhanced Raman spectroscopy (SERS) by using a nanofluidic trapping device. This nanofluidic device has a bottle neck shape composed of a microchannel leading into a nano channel that causes size-dependent trapping of nanoparticles. The analyte and Au nanoparticles, 60 nm in diameter, in aqueous solution was pumped into the channel. The nanoparticles which were larger than the narrow channel are trapped at the edge of the channel to render an enhancement of the Raman signal. We have demonstrated that the Raman scattering signal enhancement on a nanochannel-based colloidal gold cluster is able to detect 10 pM of adenine, the test analyte, without chemical modification. The efficiency and robustness of the device suggests potential for single molecule detection and multicomponent detection for biological applications and/or biotoxins.

Paper Details

Date Published: 14 February 2007
PDF: 6 pages
Proc. SPIE 6444, Ultrasensitive and Single-Molecule Detection Technologies II, 64440P (14 February 2007); doi: 10.1117/12.698759
Show Author Affiliations
I-Hsien Chou, Texas A&M Univ. (United States)
Hope T. Beier, Texas A&M Univ. (United States)
Maio Wang, Texas A&M Univ. (United States)
Nan Jing, Texas A&M Univ. (United States)
Jun Kameoka, Texas A&M Univ. (United States)
Gerard L. Coté, Texas A&M Univ. (United States)


Published in SPIE Proceedings Vol. 6444:
Ultrasensitive and Single-Molecule Detection Technologies II
Jorg Enderlein; Zygmunt K. Gryczynski, Editor(s)

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