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Proceedings Paper

Multi-modal and multi-wavelength imaging in xenografts bearing human tumor cells
Author(s): Sunkuk Kwon; Shi Ke; Wei Wang; Arlin G. Cameron; Eva Marie Sevick-Muraca
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Paper Abstract

Dynamic multi-wavelength fluorescence imaging was accomplished using a liquid crystal tunable filter (LCTF). Since several different emission wavelengths can be selected by tuning the LCTF, two wavelength dynamic fluorescence imaging was conducted in mice bearing human melanoma M21 and M21L after injection of a mixture of (i) RGD peptide conjugated with a near-infrared (NIR) dye that targeted integrin &agr;v&bgr;3 and (ii) non-specific dye, Cy5.5. Dynamic multi-wavelength imaging with LCTF can differentiate the uptake of the two different fluorescent contrast agents between tumor and normal tissue ROIs in the M21 and M21L xenograft models. Although the LCTF attenuated fluorescence signals by a factor of two when compared to holographic and bandpass filter sets used previously, Tumor to background ratio (TBR) from NIR fluorescence images with a bandpass and holographic filter were not statistically different from those acquired with the LCTF. Therefore, the benefit of spectral information as well as dynamic multi-wavelength may outweigh the impact of the lower transmission efficiencies, and could enable in vivo small animal imaging.

Paper Details

Date Published: 8 February 2007
PDF: 7 pages
Proc. SPIE 6431, Multimodal Biomedical Imaging II, 64310N (8 February 2007); doi: 10.1117/12.698750
Show Author Affiliations
Sunkuk Kwon, Baylor College of Medicine (United States)
Shi Ke, Baylor College of Medicine (United States)
Wei Wang, Baylor College of Medicine (United States)
Arlin G. Cameron, Baylor College of Medicine (United States)
Eva Marie Sevick-Muraca, Baylor College of Medicine (United States)

Published in SPIE Proceedings Vol. 6431:
Multimodal Biomedical Imaging II
Fred S. Azar, Editor(s)

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