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Proceedings Paper

Quantitative multiplex CARS spectroscopy in congested spectral regions
Author(s): Michiel Müller; Hilde A. Rinia; Mischa Bonn; Erik M. Vartiainen; Melanie Lisker; Aart van Bel
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Paper Abstract

A novel procedure is developed to describe and reproduce experimental coherent anti-Stokes Raman scattering (CARS) data, with particular emphasis on highly congested spectral regions. The approach - exemplified here with high-quality multiplex CARS data - makes use the maximum entropy method for phase retrieval. The retrieved imaginary part of the nonlinear susceptibility is shown to be equal to the spontaneous Raman spectrum. The phase retrieval procedure does not influence the noise contained in the spectra. The conversion of CARS to Raman data permits a quantitative interpretation of CARS spectra. This novel approach is demonstrated for highly congested multiplex CARS spectra of sucrose, fructose and glucose. This novel procedures enables extraction of vibrational information from multiplex CARS data without the use of a priori information of the sample.

Paper Details

Date Published: 10 February 2007
PDF: 9 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 644206 (10 February 2007); doi: 10.1117/12.698566
Show Author Affiliations
Michiel Müller, Univ. of Amsterdam (Netherlands)
Hilde A. Rinia, Univ. of Amsterdam (Netherlands)
Mischa Bonn, FOM Institute for Atomic and Molecular Physics (Netherlands)
Erik M. Vartiainen, Lappeenranta Univ. of Technology (Finland)
Melanie Lisker, Justus-Liebig-Univ. Giessen (Germany)
Aart van Bel, Justus-Liebig-Univ. Giessen (Germany)


Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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