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Proceedings Paper

Second-harmonic generation investigation of collagen thermal denaturation
Author(s): Wei-Liang Chen; Yen Sun; Sung-Jan Lin; Shiou-Hwa Jee; Yang-Fang Chen; Ling-Chih Lin; Peter T. C. So; Chen-Yuan Dong
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Paper Abstract

Using the technique of second-harmonic generation (SHG) microscopy we obtained large area image of type I collagen from rat tail tendon as it is heated from 40°C to 70°C for 0 to 180 minutes. The high resolution images allowed us to investigate the collagen structural change. We observed that heating the tendon below the temperature of 54°C does not produce any change in the averaged SHG intensity. At the heating temperature of 54°C and above, we find that increasing the heating temperature and time leads to decreasing SHG intensity. As the tendon is heated above 54°C, a decrease in the SHG signal occurs uniformly throughout the tendon, but the regions where the SHG signal vanishes form a tiger-tail like pattern. By comparing the relative SHG intensities in small and large areas, we found that the denaturation process responsible for forming the tiger-tail like pattern occurs at a higher rate than the global denaturation process occurring throughout the tendon. Our results show that second-harmonic generation microscopy is effective in monitoring the thermal damage to collagen and has potential applications in biomedicine.

Paper Details

Date Published: 10 February 2007
PDF: 8 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421D (10 February 2007); doi: 10.1117/12.698524
Show Author Affiliations
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Yen Sun, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Institute of Biomedical Engineering, National Taiwan Univ. (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Yang-Fang Chen, National Taiwan Univ. (Taiwan)
Ling-Chih Lin, National Taiwan Univ. (Taiwan)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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