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Proceedings Paper

Real-time probing of radical events with sulfide molecules
Author(s): Yann A. Gauduel; Yannick Glinec; Victor Malka
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Paper Abstract

The physio-pathological roles of sulfide biomolecules in cellular environments involves redox processes and radical reactions that alter or protect the functional properties of enzymatic systems, proteins and nucleic acids repair. We focus on micromolar monitoring of sulfur-centered radical anions produced by direct electron attachment, using sulfide molecules (a thioether and a disulfide biomolecule) and two complementary spectroscopic approaches: low energy radiation femtochemistry (1-8 eV) and high energy radiation femtochemistry (2.5-15 MeV). The early step of a disulfide bond making RS∴SR from thiol molecules involves a very-short lived odd-electron bonded intermediate for which an excess electron is transiently localized by a preexisting two sulfide monomers complex. The reactive center of oxidized glutathione (cystamine), a major cytoplasmic disulfide biomolecule, is also used as sensor for the real-time IR investigation of effective reaction radius reff in homogenous aqueous environments and interfacial water of biomimetic systems. Femtosecond high-energy electrons beams, typically in the 2.5 - 15 MeV range, may conjecture the picosecond observation of primary radical events in nanometric radiation spurs. The real-time investigation of sulfide and disulfide molecules opens exciting opportunities for sensitisation of confined environments (aqueous groove of DNA, protein pockets, sub-cellular systems) to ionizing radiation. Low and high-energy femtoradical probing foreshadow the development of new applications in radiobiology (low dose effect at the nanometric scale) and anticancer radiotherapy (pro-drogue activation).

Paper Details

Date Published: 13 February 2007
PDF: 12 pages
Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 64490E (13 February 2007); doi: 10.1117/12.698335
Show Author Affiliations
Yann A. Gauduel, Lab. d'Optique Appliquée, CNRS, Ecole Polytechnique/ENSTA (France)
Yannick Glinec, Lab. d'Optique Appliquée, CNRS, Ecole Polytechnique/ENSTA (France)
Victor Malka, Lab. d'Optique Appliquée, CNRS, Ecole Polytechnique/ENSTA (France)

Published in SPIE Proceedings Vol. 6449:
Genetically Engineered and Optical Probes for Biomedical Applications IV
Samuel Achilefu; Alexander Pavlovich Savitsky; Rebekka M. Wachter; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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