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Proceedings Paper

Visualisation of polymer distribution in polyelectrolyte microcapsules by light scattering and multiple fluorescence labelling
Author(s): J. Podskocova; D. Chorvat; G. Kollarikova; I. Lacik
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Paper Abstract

In this contribution we show application of multispectral confocal laser scanning microscopy for visualisation and characterisation of polyelectrolyte microcapsules. Fluorescent labels of different free charges, specifically bound by electrostatic interactions to the polymer chains in polyelectrolyte microcapsules, were used for questioning the spatial distribution of polymer components of the microcapsules. To separate the overlapping emission spectra of the fluorescent labels spectral imaging and linear unmixing approaches was used.

Paper Details

Date Published: 21 July 2006
PDF: 8 pages
Proc. SPIE 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, 61630V (21 July 2006); doi: 10.1117/12.697290
Show Author Affiliations
J. Podskocova, Comenius Univ. (Slovak Republic)
D. Chorvat, International Laser Ctr. (Slovak Republic)
G. Kollarikova, Polymer Institute, Slovak Academy of Sciences (Slovak Republic)
I. Lacik, Polymer Institute, Slovak Academy of Sciences (Slovak Republic)


Published in SPIE Proceedings Vol. 6163:
Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII
Valery V. Tuchin, Editor(s)

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