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Proceedings Paper

In vitro influence of hypoxia on bioluminescence imaging in brain tumor cells
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Paper Abstract

Bioluminescence Imaging (BLI) has been employed as an imaging modality to identify and characterize fundamental processes related to cancer development and response at cellular and molecular levels. This technique is based on the reaction of luciferin with oxygen in the presence of luciferase and ATP. A major concern in this technique is that tumors are generally hypoxic, either constitutively and/or as a result of treatment, therefore the oxygen available for the bioluminescence reaction could possibly be reduced to limiting levels, and thus leading to underestimation of the actual number of luciferase-labeled cells during in vivo procedures. In this report, we present the initial in vitro results of the oxygen dependence of the bioluminescence signal in rat gliosarcoma 9L cells tagged with the luciferase gene (9Lluc cells). Bioluminescence photon emission from cells exposed to different oxygen tensions was detected by a sensitive CCD camera upon exposure to luciferin. The results showed that bioluminescence signal decreased at administered pO2 levels below about 5%, falling by approximately 50% at 0.2% pO2. Additional experiments showed that changes in BLI was due to the cell inability to maintain normal levels of ATP during the hypoxic period reducing the ATP concentration to limiting levels for BLI.

Paper Details

Date Published: 13 February 2007
PDF: 8 pages
Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 64490F (13 February 2007); doi: 10.1117/12.696720
Show Author Affiliations
Eduardo H. Moriyama, Ontario Cancer Institute, Univ. of Toronto (Canada)
Mark Jarvi, Ontario Cancer Institute, Univ. of Toronto (Canada)
Mark Niedre, Ontario Cancer Institute, Univ. of Toronto (Canada)
Joseph D. Mocanu, Ontario Cancer Institute, Univ. of Toronto (Canada)
Yumi Moriyama, Ontario Cancer Institute, Univ. of Toronto (Canada)
Buhong Li, Ontario Cancer Institute, Univ. of Toronto (Canada)
Lothar Lilge, Ontario Cancer Institute, Univ. of Toronto (Canada)
Brian C. Wilson, Ontario Cancer Institute, Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 6449:
Genetically Engineered and Optical Probes for Biomedical Applications IV
Samuel Achilefu; Alexander Pavlovich Savitsky; Rebekka M. Wachter; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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