Share Email Print
cover

Proceedings Paper

Development of near-infrared fluorescent probes for nitric oxide and zinc ion
Author(s): Hirotatsu Kojima; Kazuki Kiyose; Eita Sasaki; Hiroaki Nishimatsu; Yasunobu Hirata; Tetsuo Nagano
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In fluorescence imaging studies of biological mechanisms, cyanine dyes have been employed as fluorescent labels. In particular, tricarbocyanines have the advantage that light at their emission and absorption maxima in the near-infrared (NIR) region around 650-900 nm can penetrate deeply into tissues. We successfully developed two types of cyanine dyes whose fluorescence properties change upon specific reaction with nitric oxide (NO) or zinc ion. The mechanism of fluorescence modulation of the NO probes involves photoinduced electron transfer, and the fluorescent intensity can change at the same wavelengths. We synthesized a series of amine-substituted tricarbocyanines in order to examine the correlation between the electron-donating ability of the amine and the fluorescence peak wavelength. We found that changing the electron-donating ability of the amine substituent altered the absorption and emission wavelengths. Then, we synthesized dipicolylcyanine (DIPCY), consisting of tricarbocyanine as a fluorophore and dipicolylethylenediamine as a heavy metal chelator, and investigated its response to various heavy metal ions. DIPCY can work as a ratiometric fluorescent sensor for zinc ion. This fluorescence modulation of amine-substituted tricarbocyanines should be applicable to dual-wavelength measurement of various biomolecules or enzyme activities. Thus, we have established two mechanisms for modulating the fluorescence properties of cyanines.

Paper Details

Date Published: 19 February 2007
PDF: 12 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411P (19 February 2007); doi: 10.1117/12.693047
Show Author Affiliations
Hirotatsu Kojima, The Univ. of Tokyo (Japan)
CREST, JST (Japan)
Kazuki Kiyose, The Univ. of Tokyo (Japan)
CREST, JST (Japan)
Eita Sasaki, The Univ. of Tokyo (Japan)
Hiroaki Nishimatsu, The Univ. of Tokyo (Japan)
CREST, JST (Japan)
Yasunobu Hirata, The Univ. of Tokyo (Japan)
CREST, JST (Japan)
Tetsuo Nagano, The Univ. of Tokyo (Japan)
CREST, JST (Japan)


Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

© SPIE. Terms of Use
Back to Top